YOUR PROTOCOL V2.0 // SubQ

B12 (Methylcobalamin)
Dosing Protocol

The active, methylated form of vitamin B12. Supports energy production, red blood cell formation, neurological function, and methylation pathways. Inject in a fasted state in the AM.

First time injecting? View our step-by-step Injection Tutorial before your first dose.

Concentration
5 mg/mL
Per vial
Method
Subcutaneous
Abdomen or thigh
Frequency
2 days / week
Fasted AM
Dose
50 units
Per injection
Cycle
8 weeks on
2 weeks off
Supply
2 vials
Per 8-week cycle
[01]

Dosing Schedule

Inject 50 units subcutaneously 2 times per week in a fasted state in the AM. Space your two doses evenly across the week (Monday and Thursday is the standard pattern) and keep them consistent.

Day Dose Frequency
Monday 50units 1x (fasted AM)
Tuesday Rest day
Wednesday Rest day
Thursday 50units 1x (fasted AM)
Friday Rest day
Saturday Rest day
Sunday Rest day
Tip: AM dosing matters. B12 can be energizing and may interfere with sleep if taken later in the day. Injecting fasted also improves absorption.
Do not exceed 50 units per injection. Higher doses should only be considered under provider guidance.
[02]

Cycle Structure

Run an 8-week on-cycle followed by a 2-week off-cycle. The off-period allows your body to reset. You will need 2 vials to complete one full 8-week cycle.

Cycle Timeline // 10 Weeks Total
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2
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4
5
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8
9
10

On-Cycle (8 weeks)

Off-Cycle (2 weeks)
Do not skip the off-cycle. Continuous use can lead to diminishing returns. The off-period is essential for optimal results.
Next Cycle

Ready to Reorder?

You will need 2 vials for each 8-week cycle. Order your next supply before your off-cycle ends so you're ready to start again on schedule.

Reorder B12 $85 / vial
[03]

Daily Quick Reference

Follow these steps for each injection. If this is your first time, review the Injection Tutorial for a full walkthrough.

  • 01
    Wash hands thoroughly with soap and water.
  • 02
    Draw 50 units into your syringe.
  • 03
    Clean the injection site with an alcohol swab. Rotate between abdomen and thigh.
  • 04
    Pinch the skin and insert the needle at a 45-degree angle.
  • 05
    Inject slowly to reduce any stinging sensation at the site.
  • 06
    Dispose of the syringe in a sharps container. Never recap or reuse needles.
[04]

Storage

Store your B12 vial refrigerated at 36-46°F (2-8°C) after reconstitution. Keep away from direct sunlight. Unreconstituted vials can be stored at room temperature.
[05]

Support

Questions about your protocol, dosing, or anything else? Reach our team directly at support@ivuseplus.com. We typically respond within 24 hours.

Don't run out
Order More B12 
$85/vial Reorder

This protocol is for informational purposes. Consult your healthcare provider before beginning any peptide protocol. If you experience persistent redness, swelling, or discomfort at the injection site, discontinue use and contact support.

IVUSE Protocol // B12 (Methylcobalamin) // SubQ